Dead & Company appeared on The Late Show With Stephen Colbert last night as the musical guests for the second time this week. While their performances were pre-recorded on Monday, the band’s only day off between Madison Square Garden shows, it inevitably gives fans great pleasure to see the Grateful Dead torch-holders on national television. Following Thursday’s broadcast of “Jack Straw”, the band appeared again on Friday night with a heart-warming deliverance of “Uncle John’s Band”.

In the video below, you will see Bob Weir, John Mayer, and Oteil Burbridge sharing vocals throughout the entire 1970 classic. The performance aired right after Dead & Company’s performance at TD Garden in Boston, MA on Friday night, wrapping up their two night Late Show run on the road.

Watch “Uncle John’s Band” from Friday night’s airing, and “Jack Straw” from Thursday night’s airing, below:

Dead & Company will perform at TD Garden in Boston, MA again on Sunday. For a full list of upcoming tour dates, head to the band’s website.